Dataset Information

Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani.

ABSTRACT: Sheath blight disease is caused by a necrotrophic fungal pathogen Rhizoctonia solani and it continues to be a challenge for sustainable rice cultivation. In this study, we adopted a multi-pronged approach to understand the intricacies of rice undergoing susceptible interactions with R. solani. Extensive anatomical alteration, chloroplast localized ROS, deformed chloroplast ultrastructure along with decreased photosynthetic efficiency were observed in infected tissue. GC-MS based metabolite profiling revealed accumulation of glycolysis and TCA cycle intermediates, suggesting enhanced respiration. Several aromatic and aliphatic amino acids along with phenylpropanoid intermediates were also accumulated, suggesting induction of secondary metabolism during pathogenesis. Furthermore, alterations in carbon metabolism along with perturbation of hormonal signalling were highlighted in this study. The gene expression analysis including RNAseq profiling reinforced observed metabolic alterations in the infected tissues. In conclusion, the present study unravels key events associated during susceptible rice-R. solani interactions and identifies metabolites and transcripts that are accumulated in infected tissues.

SUBMITTER: Ghosh S

PROVIDER: S-EPMC5292701 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani.

Ghosh Srayan S Kanwar Poonam P Jha Gopaljee G

Scientific reports 20170206

Sheath blight disease is caused by a necrotrophic fungal pathogen Rhizoctonia solani and it continues to be a challenge for sustainable rice cultivation. In this study, we adopted a multi-pronged approach to understand the intricacies of rice undergoing susceptible interactions with R. solani. Extensive anatomical alteration, chloroplast localized ROS, deformed chloroplast ultrastructure along with decreased photosynthetic efficiency were observed in infected tissue. GC-MS based metabolite profi ...[more]

PMID: 28165003

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Project description:Rice is a staple food crop and is a major source of employment and income in the world. However, the attack of fungal disease poses a serious threat to the crop growth and productivity and leads toward yield loses. Therefore, current study was performed to evaluate the biocontrol potential of Lactobacillus and Weissella spp. on basmati rice against Rhizoctonia solani. Agar disc method was performed to evaluate the antifungal activity of both bacteria against R. solani. Petri plate and pot experiments were conducted to evaluate the growth promotion and biocontrol potential of both bacteria in Basmati rice under R. solani stress. Results indicated that maximum antifungal activity (82%) was recorded by Lactobacillus sp. Maximum phosphate solubilization and siderophore production was recorded by Weissella sp. In petri plate experiment, maximum root length, root fresh and dry weight (36%, 40% and 13%) was recorded by Weissella sp. and maximum shoot length and shoot fresh weight (99% and 107%) by Lactobacillus sp. In pot experiment, both bacteria enhanced the growth parameters of Basmati rice including root and shoot length, fresh and dry weight as well as no. of lateral roots. Application of Weissella sp. resulted in maximum increase (332% and 134%) in chlorophyll a and b content while Lactobacillus sp. + R. solani showed maximum (42%) carotenoid contents. Lactobacillus sp. + R. solani showed maximum increase in the proline (54%) and sugar contents (100%) while Lactobacillus sp. alone showed maximum (35%) soluble protein contents. Plant defense enzymes i-e SOD (400%), POD (25%), CAT (650%), PPO (14%) and PAL (124%) were notably increased by Weissella sp. + R. solani and Lactobacillus sp + R. solani. The Lactobacillus sp showed the best results in antifungal activity against R. solani and Weissella sp. showed the best results in production of defense enzymes in basmati rice against R. solani stress and can be suggested as the potent biocontrol agents for the rice crop.

Dataset Information

Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani.

Publications

Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani.

Similar Datasets

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