Ontology highlight
ABSTRACT:
SUBMITTER: Grison M
PROVIDER: S-EPMC5293040 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Grison Marco M Merkel Ulrich U Kostan Julius J Djinović-Carugo Kristina K Rief Matthias M
Proceedings of the National Academy of Sciences of the United States of America 20170117 5
Stable anchoring of titin within the muscle Z-disk is essential for preserving muscle integrity during passive stretching. One of the main candidates for anchoring titin in the Z-disk is the actin cross-linker α-actinin. The calmodulin-like domain of α-actinin binds to the Z-repeats of titin. However, the mechanical and kinetic properties of this important interaction are still unknown. Here, we use a dual-beam optical tweezers assay to study the mechanics of this interaction at the single-molec ...[more]