Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase.

ABSTRACT:

Background

Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible to culture.

Objective

In this study, we aimed to standardise an in situ hybridisation technique for the differentiation between the pathogenic species Paracoccidioides brasiliensis and Paracoccidioides lutzii, by using species-specific DNA probes targeting the internal transcribed spacer-1 (ITS-1) of the rRNA gene.

Methods

Yeast and mycelial phase of each Paracoccidioides species, were tested by two different detecti

SUBMITTER: Arantes TD 

PROVIDER: S-EPMC5293123 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature