High Affinity vs. Native Fibronectin in the Modulation of ?v?3 Integrin Conformational Dynamics: Insights from Computational Analyses and Implications for Molecular Design.

ABSTRACT: Understanding how binding events modulate functional motions of multidomain proteins is a major issue in chemical biology. We address several aspects of this problem by analyzing the differential dynamics of ?v?3 integrin bound to wild type (wtFN10, agonist) or high affinity (hFN10, antagonist) mutants of fibronectin. We compare the dynamics of complexes from large-scale domain motions to inter-residue coordinated fluctuations to characterize the distinctive traits of conformational evolution and shed light on the determinants of differential ?v?3 activation induced by different FN sequences. We propose an allosteric model for ligand-based integrin modulation: the conserved integrin binding pocket anchors the ligand, while different residues on the two FN10's act as the drivers that reorganize relevant interaction networks, guiding the shift towards inactive (hFN10-bound) or active states (wtFN10-bound). We discuss the implications of results for the design of integrin inhibitors.

SUBMITTER: Paladino A 

PROVIDER: S-EPMC5293283 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature