Dataset Information

Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca²⁺-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.

ABSTRACT: We previously reported that eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid (n-3 PUFA), effectively inhibits sphingosylphosphorylcholine (SPC)-induced Ca²⁺-sensitization of vascular smooth muscle (VSM) contraction which is a major cause of cardiovascular and cerebrovascular vasospasm, and EPA is utilized clinically to prevent cerebrovascular vasospasm. In this study, we clearly demonstrate that docosapentaenoic acid (DPA), which exists in two forms as omega-3 (n-3) and omega-6 (n-6) PUFA, strongly inhibits SPC-induced contraction in VSM tissue and human coronary artery smooth muscle cells (CASMCs), with little effect on Ca²⁺-dependent contraction. Furthermore, n-3 and n-6 DPA inhibited the activation and translocation of Rho-kinase from cytosol to cell membrane. Additionally, SPC-induced phosphorylation of myosin light chain (MLC) was inhibited in n-3 and n-6 DPA pretreated smooth muscleVSM cells and tissues. In summary, we provide direct evidence that n-3 and n-6 DPA effectively equally inhibits SPC-induced contraction by inhibiting Rho-kinase activation and translocation to the cell membrane.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC5294466 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca<sup>2+</sup>-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.

Zhang Ying Y Zhang Min M Lyu Bochao B Kishi Hiroko H Kobayashi Sei S

Scientific reports 20170207

We previously reported that eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid (n-3 PUFA), effectively inhibits sphingosylphosphorylcholine (SPC)-induced Ca<sup>2+</sup>-sensitization of vascular smooth muscle (VSM) contraction which is a major cause of cardiovascular and cerebrovascular vasospasm, and EPA is utilized clinically to prevent cerebrovascular vasospasm. In this study, we clearly demonstrate that docosapentaenoic acid (DPA), which exists in two forms as omega-3 (n-3) ...[more]

PMID: 28169288

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Dataset Information

Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca²⁺-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.

Publications

Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca<sup>2+</sup>-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.

Similar Datasets

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Dataset Information

Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca2+-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.

Publications

Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca<sup>2+</sup>-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca²⁺-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.