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Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08.

ABSTRACT: Unsaturated alginate disaccharides (UADs), enzymatically derived from the degradation of alginate polymers, are considered powerful antioxidants. In this study, a new high UAD-producing alginate lyase, AlySY08, has been purified from the marine bacterium Vibrio sp. SY08. AlySY08, with a molecular weight of about 33 kDa and a specific activity of 1070.2 U/mg, showed the highest activity at 40 °C in phosphate buffer at pH 7.6. The enzyme was stable over a broad pH range (6.0-9.0) and retained about 75% activity after incubation at 40 °C for 2 h. Moreover, the enzyme was active in the absence of salt ions and its activity was enhanced by the addition of NaCl and KCl. AlySY08 resulted in an endo-type alginate lyase that degrades both polyM and polyG blocks, yielding UADs as the main product (81.4% of total products). All these features made AlySY08 a promising candidate for industrial applications in the production of antioxidants from alginate polysaccharides.

SUBMITTER: Li S

PROVIDER: S-EPMC5295221 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp. SY08.

Li Shangyong S Wang Linna L Hao Jianhua J Xing Mengxin M Sun Jingjing J Sun Mi M

Marine drugs 20161223 1

Unsaturated alginate disaccharides (UADs), enzymatically derived from the degradation of alginate polymers, are considered powerful antioxidants. In this study, a new high UAD-producing alginate lyase, AlySY08, has been purified from the marine bacterium <i>Vibrio</i> sp. SY08. AlySY08, with a molecular weight of about 33 kDa and a specific activity of 1070.2 U/mg, showed the highest activity at 40 °C in phosphate buffer at pH 7.6. The enzyme was stable over a broad pH range (6.0-9.0) and retain ...[more]

PMID: 28025527

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