Ontology highlight
ABSTRACT:
SUBMITTER: Pilgrim MG
PROVIDER: S-EPMC5295770 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Pilgrim Matthew G MG Lengyel Imre I Lanzirotti Antonio A Newville Matt M Fearn Sarah S Emri Eszter E Knowles Jonathan C JC Messinger Jeffrey D JD Read Russell W RW Guidry Clyde C Curcio Christine A CA
Investigative ophthalmology & visual science 20170201 2
<h4>Purpose</h4>Extracellular deposits containing hydroxyapatite, lipids, proteins, and trace metals that form between the basal lamina of the RPE and the inner collagenous layer of Bruch's membrane are hallmarks of early AMD. We examined whether cultured RPE cells could produce extracellular deposits containing all of these molecular components.<h4>Methods</h4>Retinal pigment epithelium cells isolated from freshly enucleated porcine eyes were cultured on Transwell membranes for up to 6 months. ...[more]