Dataset Information

Modified forelimb grip strength test detects aging-associated physiological decline in skeletal muscle function in male mice.

ABSTRACT: The conventional forelimb grip strength test is a widely used method to assess skeletal muscle function in rodents; in this study, we modified this method to improve its variability and consistency. The modified test had lower variability among trials and days than the conventional test in young C57BL6 mice, especially by improving the variabilities in male. The modified test was more sensitive than the conventional test to detect a difference in motor function between female and male mice, or between young and old male mice. When the modified test was performed on male mice during the aging process, reduction of grip strength manifested between 18 and 24 months of age at the group level and at the individual level. The modified test was similar to the conventional test in detecting skeletal muscle dysfunction in young male dystrophic mice. Thus, the modified forelimb grip strength test, with its improved validity and reliability may be an ideal substitute for the conventional method.

SUBMITTER: Takeshita H

PROVIDER: S-EPMC5296723 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Modified forelimb grip strength test detects aging-associated physiological decline in skeletal muscle function in male mice.

Takeshita Hikari H Yamamoto Koichi K Nozato Satoko S Inagaki Tadakatsu T Tsuchimochi Hirotsugu H Shirai Mikiyasu M Yamamoto Ryohei R Imaizumi Yuki Y Hongyo Kazuhiro K Yokoyama Serina S Takeda Masao M Oguro Ryosuke R Takami Yoichi Y Itoh Norihisa N Takeya Yasushi Y Sugimoto Ken K Fukada So-Ichiro SI Rakugi Hiromi H

Scientific reports 20170208

The conventional forelimb grip strength test is a widely used method to assess skeletal muscle function in rodents; in this study, we modified this method to improve its variability and consistency. The modified test had lower variability among trials and days than the conventional test in young C57BL6 mice, especially by improving the variabilities in male. The modified test was more sensitive than the conventional test to detect a difference in motor function between female and male mice, or b ...[more]

PMID: 28176863

Similar Datasets

Project description:BackgroundUpper and lower extremity muscle strength can be used to predict health outcomes. However, the difference between the relation of upper extremity muscle and of lower extremity muscle with physiological factors is unclear. This study aimed to evaluate the association between physiological data and muscle strength, measured using grip and leg extension strength, among Japanese adults.MethodsWe conducted a cross-sectional study of 2,861 men and 6,717 women aged ≥ 20 years living in Miyagi Prefecture, Japan. Grip strength was measured using a dynamometer. Leg extension strength was measured using a hydraulic isokinetic leg press machine. Anthropometry and physiological data, including blood pressure, calcaneal ultrasound bone status, pulmonary function, carotid echography, and blood information, were assessed. We used a general linear model adjusted for age, body composition, and smoking status to evaluate the association between muscle strength and physiological factors.ResultsGrip and leg extension strength were positively associated with bone area ratio, vital capacity, forced vital capacity, forced expiratory volume in one second, and estimated glomerular filtration rate, and negatively associated with waist circumference and percentage body fat mass in both the sexes. Diastolic blood pressure was positively associated with grip strength in both the sexes and leg extension strength in men, but not women. High-density lipoprotein cholesterol and red blood cell counts were positively associated with grip and leg extension strength in women, but not men. In both the sexes, pulse rate, total cholesterol, and uric acid were consistently associated with only leg extension strength, but not grip strength. In women, glycated hemoglobin demonstrated negative and positive associations with grip and leg extension strength, respectively.ConclusionsGrip and leg extension strength demonstrated similar associations with anthropometry, pulmonary function, and estimated glomerular filtration rate, but the associations with the other factors were not always consistent.

Dataset Information

Modified forelimb grip strength test detects aging-associated physiological decline in skeletal muscle function in male mice.

Publications

Modified forelimb grip strength test detects aging-associated physiological decline in skeletal muscle function in male mice.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets