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Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection.

ABSTRACT: Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL^-1 with a dynamic range of 1-200 ng mL^-1. Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.

SUBMITTER: Zhu X

PROVIDER: S-EPMC5298300 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection.

Zhu Xuena X Sarwar Mehenur M Yue Qiaoli Q Chen Chunying C Li Chen-Zhong CZ

International journal of nanomedicine 20170202

Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL ...[more]

PMID: 28203077

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