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Chemical Protein Ubiquitylation with Preservation of the Native Cysteine Residues.

ABSTRACT: We report a cysteine-based ligation strategy for generating a monoubiquitylated protein while preserving the native cysteine residues on the acceptor protein. In monoubiquitylation of proliferating cell nuclear antigen (PCNA) this method circumvents the need to mutate the native cysteine residues on PCNA. The chemically ubiquitylated PCNA contains a noncleavable linkage of the same length as the native isopeptide linkage. It also retains the normal function of the native Ub-PCNA in stimulating the ATPase activity of replication factor?C (RFC) and lesion bypass synthesis by Pol?. This method may be adapted for chemical ubiquitylation of other proteins and for site-specific modification of a target protein at a specific site through sulfhydryl chemistry.

SUBMITTER: Yang K

PROVIDER: S-EPMC5298353 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Chemical Protein Ubiquitylation with Preservation of the Native Cysteine Residues.

Yang Kun K Li Guorui G Gong Ping P Gui Weijun W Yuan Libo L Zhuang Zhihao Z

Chembiochem : a European journal of chemical biology 20160426 11

We report a cysteine-based ligation strategy for generating a monoubiquitylated protein while preserving the native cysteine residues on the acceptor protein. In monoubiquitylation of proliferating cell nuclear antigen (PCNA) this method circumvents the need to mutate the native cysteine residues on PCNA. The chemically ubiquitylated PCNA contains a noncleavable linkage of the same length as the native isopeptide linkage. It also retains the normal function of the native Ub-PCNA in stimulating t ...[more]

PMID: 27113245

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