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Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging.

ABSTRACT: Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application of a new class of large Stokes shift fluorescent dyes that are water-soluble, cell permeable, non-cytotoxic, and lysosome-specific. Additionally, we demonstrate temporally controlled sequential photoactivation of individual cells in close spatial proximity.

SUBMITTER: Tran MN

PROVIDER: S-EPMC5299591 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging.

Tran Mai N MN Rarig Robert-André F RF Chenoweth David M DM

Chemical science 20150617 8

Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application of a new class of large Stokes shift fluorescent dyes that are water-soluble, cell permeable, non-cytotoxic, and lysosome-specific. Additionally, we demonstrate temporally controlled sequential photoa ...[more]

PMID: 28496967

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