Ontology highlight
ABSTRACT:
SUBMITTER: Fernandez-Leiro R
PROVIDER: S-EPMC5300136 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Fernandez-Leiro Rafael R Conrad Julian J Yang Ji-Chun JC Freund Stefan M V SM Scheres Sjors H W SH Lamers Meindert H MH
Nature structural & molecular biology 20170109 2
Faithful DNA replication is essential to all forms of life and depends on the action of 3'-5' exonucleases that remove misincorporated nucleotides from the newly synthesized strand. However, how the DNA is transferred from the polymerase to the exonuclease active site is not known. Here we present the cryo-EM structure of the editing mode of the catalytic core of the Escherichia coli replisome, revealing a dramatic distortion of the DNA whereby the polymerase thumb domain acts as a wedge that se ...[more]