Interference between Triplex and Protein Binding to Distal Sites on Supercoiled DNA.
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ABSTRACT:
SUBMITTER: Noy A
PROVIDER: S-EPMC5300792 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Publications
Interference between Triplex and Protein Binding to Distal Sites on Supercoiled DNA.
Noy Agnes A Maxwell Anthony A Harris Sarah A SA
Biophysical journal 20170117 3
We have explored the interdependence of the binding of a DNA triplex and a repressor protein to distal recognition sites on supercoiled DNA minicircles using MD simulations. We observe that the interaction between the two ligands through their influence on their DNA template is determined by a subtle interplay of DNA mechanics and electrostatics, that the changes in flexibility induced by ligand binding play an important role and that supercoiling can instigate additional ligand-DNA contacts tha ...[more]
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