Ontology highlight
ABSTRACT:
SUBMITTER: Chang X
PROVIDER: S-EPMC5302446 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Chang Xiaoyu X Xu Bo B Bai Yingguo Y Luo Huiying H Ma Rui R Shi Pengjun P Yao Bin B
PloS one 20170210 2
N-Glycosylation is a posttranslational modification commonly occurred in fungi and plays roles in a variety of enzyme functions. In this study, a xylanase (Af-XYNA) of glycoside hydrolase (GH) family 10 from Aspergillus fumigatus harboring three potential N-glycosylation sites (N87, N124 and N335) was heterologously produced in Pichia pastoris. The N-glycosylated Af-XYNA (WT) exhibited favorable temperature and pH optima (75°C and pH 5.0) and good thermostability (maintaining stable at 60°C). To ...[more]