Dataset Information

Re-assessing gallium-67 as a therapeutic radionuclide.

ABSTRACT:

Introduction

Despite its desirable half-life and low energy Auger electrons that travel further than for other radionuclides, ⁶⁷Ga has been neglected as a therapeutic radionuclide. Here, ⁶⁷Ga is compared with Auger electron emitter ¹¹¹In as a potential therapeutic radionuclide.

Methods

Plasmid pBR322 studies allowed direct comparison between ⁶⁷Ga and ¹¹¹In (1MBq) in causing DNA damage, including the effect of chelators (EDTA and DTPA) and the effects of a free radical scavenger (DMSO). The cytotoxicity of internalized (by means of delivery in the form of oxine complexes) and non-internalized ⁶⁷Ga and ¹¹¹In was measured in DU145 prostate cancer cells after a one-hour incubation using cell viability (trypan blue) and clonogenic studies. MDA-MB-231 and HCC1954 cells were also used.

Results

Plasmid DNA damage was caused by ⁶⁷Ga and was comparable to that caused by ¹¹¹In; it was reduced in the presence of EDTA, DTPA and DMSO. The A₅₀ values (internalized activity of oxine complexes per cell required to kill 50% of cells) as determined by trypan blue staining was 1.0Bq/cell for both ⁶⁷Ga and ¹¹¹In; the A₅₀ values determined by clonogenic assay were 0.7Bq/cell and 0.3Bq/cell for ¹¹¹In and ⁶⁷Ga respectively. At the concentrations required to achieve these uptake levels, non-internalized ⁶⁷Ga and ¹¹¹In caused no cellular toxicity. Qualitatively similar results were found for MDA-MB-231 and HCC1954 cells.

Conclusion

⁶⁷Ga causes as much damage as ¹¹¹In to plasmid DNA in solution and shows similar toxicity as ¹¹¹In at equivalent internalized activity per cell. ⁶⁷Ga therefore deserves further evaluation for radionuclide therapy.

Advances in knowledge and implications for patient care

The data presented here is at the basic level of science. If future in vivo and clinical studies are successful, ⁶⁷Ga could become a useful radionuclide with little healthy tissue toxicity in the arsenal of weapons for treating cancer.

SUBMITTER: Othman MF

PROVIDER: S-EPMC5303015 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Publications

Re-assessing gallium-67 as a therapeutic radionuclide.

Othman Muhamad F Bin MF Mitry Nabil R NR Lewington Valerie J VJ Blower Philip J PJ Terry Samantha Y A SY

Nuclear medicine and biology 20161029

<h4>Introduction</h4>Despite its desirable half-life and low energy Auger electrons that travel further than for other radionuclides, <sup>67</sup>Ga has been neglected as a therapeutic radionuclide. Here, <sup>67</sup>Ga is compared with Auger electron emitter <sup>111</sup>In as a potential therapeutic radionuclide.<h4>Methods</h4>Plasmid pBR322 studies allowed direct comparison between <sup>67</sup>Ga and <sup>111</sup>In (1MBq) in causing DNA damage, including the effect of chelators (EDTA a ...[more]

PMID: 27915165

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Dataset Information

Re-assessing gallium-67 as a therapeutic radionuclide.

Introduction

Methods

Results

Conclusion

Advances in knowledge and implications for patient care

Publications

Re-assessing gallium-67 as a therapeutic radionuclide.

Similar Datasets

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