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Harvesting pre-polarized macrophages using thermo-responsive substrates.

ABSTRACT: In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whilst having a minimal effect on the cell phenotype.

SUBMITTER: Malheiro V

PROVIDER: S-EPMC5307341 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Harvesting pre-polarized macrophages using thermo-responsive substrates.

Malheiro Vera V Elbs-Glatz Yvonne Y Obarzanek-Fojt Magdalena M Maniura-Weber Katharina K Bruinink Arie A

Scientific reports 20170214

In the cell culture environment macrophages are highly adherent cells. Currently used methods to harvest macrophages have the disadvantage of reducing cell viability and their ability to re-attach after seeding. Although thermo-responsive surfaces have been employed to harvest cell sheets no reports are available to use these to harvest (pre-polarized) macrophages. We show that this method significantly improves the yield of living macrophages and percentage of subsequent cell reattachment, whil ...[more]

PMID: 28195152

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