Dataset Information

Circulating classical monocytes are associated with CD11c⁺ macrophages in human visceral adipose tissue.

ABSTRACT: Immune cell accumulation in adipose tissue (AT) is associated with the development of AT inflammation, resulting in metabolic dysfunction. Circulating immune cell patterns may reflect immune cell accumulation in expanding AT. However, data linking human leukocytes in blood and AT is lacking. We investigated whether blood immune cell populations are associated with their counterparts in subcutaneous (scAT) or visceral AT (vAT). Flow cytometry was performed on blood, scAT and vAT from 16 lean and 29 obese men. Circulating natural killer (NK)-cells, classical monocytes and nonclassical monocytes were higher in obese individuals. vAT, but not scAT, of obese individuals contained more inflammatory CD11c⁺ "M1" macrophages and NK cells compared to lean individuals. Blood classical monocytes were associated with CD11c⁺ macrophages in vAT but not scAT. This association was unrelated to expression of the adhesion molecules CD11b and CD11c or of the chemokine receptor CX3CR1 on these monocytes. Other AT immune cells were not associated with their respective counterparts in blood. Finally, CD11c⁺ macrophages and CD4⁺ T-cells in vAT were associated with their counterparts in scAT. In conclusion, blood classical monocytes reflect CD11c⁺ macrophages in vAT.

SUBMITTER: Wouters K

PROVIDER: S-EPMC5309742 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Publications

Circulating classical monocytes are associated with CD11c<sup>+</sup> macrophages in human visceral adipose tissue.

Wouters Kristiaan K Gaens Katrien K Bijnen Mitchell M Verboven Kenneth K Jocken Johan J Wetzels Suzan S Wijnands Erwin E Hansen Dominique D van Greevenbroek Marleen M Duijvestijn Adriaan A Biessen Erik A L EA Blaak Ellen E EE Stehouwer Coen D A CD Schalkwijk Casper G CG

Scientific reports 20170215

Immune cell accumulation in adipose tissue (AT) is associated with the development of AT inflammation, resulting in metabolic dysfunction. Circulating immune cell patterns may reflect immune cell accumulation in expanding AT. However, data linking human leukocytes in blood and AT is lacking. We investigated whether blood immune cell populations are associated with their counterparts in subcutaneous (scAT) or visceral AT (vAT). Flow cytometry was performed on blood, scAT and vAT from 16 lean and ...[more]

PMID: 28198418

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Project description:Obesity is a complex multifactorial phenotype that influences several metabolic pathways. Yet, few studies have examined the relations of different body fat compartments to urinary and serum metabolites. Anthropometric phenotypes (visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), the ratio between VAT and SAT (VSR), body mass index (BMI), waist circumference (WC)) and urinary and serum metabolite concentrations measured by nuclear magnetic resonance spectroscopy were measured in a population-based sample of 228 healthy adults. Multivariable linear and logistic regression models, corrected for multiple testing using the false discovery rate, were used to associate anthropometric phenotypes with metabolites. We adjusted for potential confounding variables: age, sex, smoking, physical activity, menopausal status, estimated glomerular filtration rate (eGFR), urinary glucose, and fasting status. In a fully adjusted logistic regression model dichotomized for the absence or presence of quantifiable metabolite amounts, VAT, BMI and WC were inversely related to urinary choline (ß = -0.18, p = 2.73*10-3), glycolic acid (ß = -0.20, 0.02), and guanidinoacetic acid (ß = -0.12, p = 0.04), and positively related to ethanolamine (ß = 0.18, p = 0.02) and dimethylamine (ß = 0.32, p = 0.02). BMI and WC were additionally inversely related to urinary glutamine and lactic acid. Moreover, WC was inversely associated with the detection of serine. VAT, but none of the other anthropometric parameters, was related to serum essential amino acids, such as valine, isoleucine, and phenylalanine among men. Compared to other adiposity measures, VAT demonstrated the strongest and most significant relations to urinary and serum metabolites. The distinct relations of VAT, SAT, VSR, BMI, and WC to metabolites emphasize the importance of accurately differentiating between body fat compartments when evaluating the potential role of metabolic regulation in the development of obesity-related diseases, such as insulin resistance, type 2 diabetes, and cardiovascular disease.

Dataset Information

Circulating classical monocytes are associated with CD11c⁺ macrophages in human visceral adipose tissue.

Publications

Circulating classical monocytes are associated with CD11c<sup>+</sup> macrophages in human visceral adipose tissue.

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Dataset Information

Circulating classical monocytes are associated with CD11c+ macrophages in human visceral adipose tissue.

Publications

Circulating classical monocytes are associated with CD11c<sup>+</sup> macrophages in human visceral adipose tissue.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Circulating classical monocytes are associated with CD11c⁺ macrophages in human visceral adipose tissue.