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C/EBP-Induced Transdifferentiation Reveals Granulocyte-Macrophage Precursor-like Plasticity of B Cells.

ABSTRACT: The lymphoid-myeloid transdifferentiation potentials of members of the C/EBP family (C/EBP?, ?, ?, and ?) were compared in v-Abl-immortalized primary B cells. Conversion of B cells to macrophages was readily induced by the ectopic expression of any C/EBP, and enhanced by endogenous C/EBP? and ? activation. High transgene expression of C/EBP? or C/EBP?, but not of C/EBP? or C/EBP?, also induced the formation of granulocytes. Granulocytes and macrophages emerged in a mutually exclusive manner. C/EBP?-expressing B cells produced granulocyte-macrophage progenitor (GMP)-like progenitors when subjected to selective pressure to eliminate lymphoid cells. The GMP-like progenitors remained self-renewing and cytokine-independent, and continuously produced macrophages and granulocytes. In addition to their suitability to study myelomonocytic lineage bifurcation, lineage-switched GMP-like progenitors could reflect the features of the lympho-myeloid lineage switch observed in leukemic progression.

SUBMITTER: Cirovic B 

PROVIDER: S-EPMC5312250 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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C/EBP-Induced Transdifferentiation Reveals Granulocyte-Macrophage Precursor-like Plasticity of B Cells.

Cirovic Branko B   Schönheit Jörg J   Kowenz-Leutz Elisabeth E   Ivanovska Jelena J   Klement Christine C   Pronina Nina N   Bégay Valérie V   Leutz Achim A  

Stem cell reports 20170119 2

The lymphoid-myeloid transdifferentiation potentials of members of the C/EBP family (C/EBPα, β, δ, and ε) were compared in v-Abl-immortalized primary B cells. Conversion of B cells to macrophages was readily induced by the ectopic expression of any C/EBP, and enhanced by endogenous C/EBPα and β activation. High transgene expression of C/EBPβ or C/EBPε, but not of C/EBPα or C/EBPδ, also induced the formation of granulocytes. Granulocytes and macrophages emerged in a mutually exclusive manner. C/E  ...[more]

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