Ontology highlight
ABSTRACT:
SUBMITTER: Wang B
PROVIDER: S-EPMC5313047 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Cell reports 20170201 6
Intrinsic resistance and RTK-RAS-MAPK pathway reactivation has limited the effectiveness of MEK and RAF inhibitors (MAPKi) in RAS- and RAF-mutant cancers. To identify genes that modulate sensitivity to MAPKi, we performed genome-scale CRISPR-Cas9 loss-of-function screens in two KRAS mutant pancreatic cancer cell lines treated with the MEK1/2 inhibitor trametinib. Loss of CIC, a transcriptional repressor of ETV1, ETV4, and ETV5, promoted survival in the setting of MAPKi in cancer cells derived fr ...[more]