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Proteomic analysis of urinary extracellular vesicles from high Gleason score prostate cancer.

ABSTRACT: Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. EVs were isolated from urine after massage from 18 men (negative biopsy [n?=?6], GS 6 PCa [n?=?6], or GS 8-9 PCa [n?=?6]). EV proteins were labeled with iTRAQ and analyzed by LC-MS/MS. We identified 4710 proteins and quantified 3528 proteins in the urinary EVs. Eleven proteins increased in patients with PCa compared to those with negative biopsy (ratio >1.5, p-value?

SUBMITTER: Fujita K

PROVIDER: S-EPMC5314323 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Proteomic analysis of urinary extracellular vesicles from high Gleason score prostate cancer.

Fujita Kazutoshi K Kume Hideaki H Matsuzaki Kyosuke K Kawashima Atsunari A Ujike Takeshi T Nagahara Akira A Uemura Motohide M Miyagawa Yasushi Y Tomonaga Takeshi T Nonomura Norio N

Scientific reports 20170217

Extracellular vesicles (EVs) are microvesicles secreted from various cell types. We aimed to discover a new biomarker for high Gleason score (GS) prostate cancer (PCa) in urinary EVs via quantitative proteomics. EVs were isolated from urine after massage from 18 men (negative biopsy [n = 6], GS 6 PCa [n = 6], or GS 8-9 PCa [n = 6]). EV proteins were labeled with iTRAQ and analyzed by LC-MS/MS. We identified 4710 proteins and quantified 3528 proteins in the urinary EVs. Eleven proteins increased ...[more]

PMID: 28211531

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