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Multimodality Imaging of Bone Marrow-Derived Dendritic Cell Migration and Antitumor Immunity.


ABSTRACT: Here, we sought to monitor bone marrow-derived dendritic cell (BMDC) migration and antitumor effects using a multimodal reporter imaging strategy in living mice. BMDCs were transduced with retroviral vector harboring human sodium iodide symporter (hNIS, nuclear imaging reporter), firefly luc2 (optical imaging reporter), and thy1.1 (surrogate marker of NIS and luc2) genes (BMDC/NF cells). No significant differences in biological functions, including cell proliferation, antigen uptake, phenotype expression, and migration ability, were observed between BMDC and BMDC/NF cells. Combined bioluminescence imaging and I-124 positron emission tomography/computed tomography clearly revealed the migration of BMDC/NF cells to draining popliteal lymph nodes at day 7 postinjection. Interestingly, marked tumor protection was observed in mice immunized with TC-1 lysate-pulsed BMDC/NF cells. Our findings suggested that multimodal reporter gene imaging of NIS and luciferase could provide insights into the biological behaviors of dendritic cells in living organisms and could be a useful tool for the optimization of DC-based immunotherapy protocols.

SUBMITTER: Ahn SB 

PROVIDER: S-EPMC5314440 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Multimodality Imaging of Bone Marrow-Derived Dendritic Cell Migration and Antitumor Immunity.

Ahn Su-Bi SB   Lee Sang Bong SB   Singh Thoudam Debraj TD   Cho Sung Jin SJ   Kim Sang Kyoon SK   Lee In-Kyu IK   Jeong Shin Young SY   Ahn Byeong-Cheol BC   Lee Jaetae J   Lee Sang-Woo SW   Jeon Yong Hyun YH  

Translational oncology 20170216 2


Here, we sought to monitor bone marrow-derived dendritic cell (BMDC) migration and antitumor effects using a multimodal reporter imaging strategy in living mice. BMDCs were transduced with retroviral vector harboring human sodium iodide symporter (hNIS, nuclear imaging reporter), firefly luc2 (optical imaging reporter), and thy1.1 (surrogate marker of NIS and luc2) genes (BMDC/NF cells). No significant differences in biological functions, including cell proliferation, antigen uptake, phenotype e  ...[more]

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