Ontology highlight
ABSTRACT:
SUBMITTER: Whitley KD
PROVIDER: S-EPMC5314771 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Whitley Kevin D KD Comstock Matthew J MJ Chemla Yann R YR
Nucleic acids research 20161129 2
Despite its fundamental importance in cellular processes and abundant use in biotechnology, we lack a detailed understanding of the kinetics of nucleic acid hybridization. In particular, the identity of the transition state, which determines the kinetics of the two-state reaction, remains poorly characterized. Here, we used optical tweezers with single-molecule fluorescence to observe directly the binding and unbinding of short oligonucleotides (7-12 nt) to a complementary strand held under cons ...[more]