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Identification of downy mildew resistance gene candidates by positional cloning in maize (Zea mays subsp. mays; Poaceae).


ABSTRACT: PREMISE OF THE STUDY:Positional cloning in combination with phenotyping is a general approach to identify disease-resistance gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combined strategy to improve the identification of disease-resistance gene candidates. METHODS AND RESULTS:Downy mildew (DM)-resistant maize was selected from five cultivars using a spreader row technique. Positional cloning and bioinformatics tools were used to identify the DM-resistance quantitative trait locus marker (bnlg1702) and 47 protein-coding gene annotations. Eventually, five DM-resistance gene candidates, including bZIP34, Bak1, and Ppr, were identified by quantitative reverse-transcription PCR (RT-PCR) without fine mapping of the bnlg1702 locus. CONCLUSIONS:The combined protocol with the spreader row technique, quantitative trait locus positional cloning, and quantitative RT-PCR was effective for identifying DM-resistance candidate genes. This cloning approach may be applied to other whole-genome-sequenced crops or resistance to other diseases.

SUBMITTER: Kim JY 

PROVIDER: S-EPMC5315382 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Identification of downy mildew resistance gene candidates by positional cloning in maize (<i>Zea mays</i> subsp. <i>mays</i>; Poaceae).

Kim Jae Yoon JY   Moon Jun-Cheol JC   Kim Hyo Chul HC   Shin Seungho S   Song Kitae K   Kim Kyung-Hee KH   Lee Byung-Moo BM  

Applications in plant sciences 20170214 2


<h4>Premise of the study</h4>Positional cloning in combination with phenotyping is a general approach to identify disease-resistance gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combined strategy to improve the identification of disease-resistance gene candidates.<h4>Methods and results</h4>Downy mildew (DM)-resistant maize was selected from five cultivars using a spreader row  ...[more]

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