Dataset Information

Genome-wide identification of direct HBx genomic targets.

ABSTRACT:

Background

The Hepatitis B Virus (HBV) HBx regulatory protein is required for HBV replication and involved in HBV-related carcinogenesis. HBx interacts with chromatin modifying enzymes and transcription factors to modulate histone post-translational modifications and to regulate viral cccDNA transcription and cellular gene expression. Aiming to identify genes and non-coding RNAs (ncRNAs) directly targeted by HBx, we performed a chromatin immunoprecipitation sequencing (ChIP-Seq) to analyse HBV recruitment on host cell chromatin in cells replicating HBV.

Results

ChIP-Seq high throughput sequencing of HBx-bound fragments was used to obtain a high-resolution, unbiased, mapping of HBx binding sites across the genome in HBV replicating cells. Protein-coding genes and ncRNAs involved in cell metabolism, chromatin dynamics and cancer were enriched among HBx targets together with genes/ncRNAs known to modulate HBV replication. The direct transcriptional activation of genes/miRNAs that potentiate endocytosis (Ras-related in brain (RAB) GTPase family) and autophagy (autophagy related (ATG) genes, beclin-1, miR-33a) and the transcriptional repression of microRNAs (miR-138, miR-224, miR-576, miR-596) that directly target the HBV pgRNA and would inhibit HBV replication, contribute to HBx-mediated increase of HBV replication.

Conclusions

Our ChIP-Seq analysis of HBx genome wide chromatin recruitment defined the repertoire of genes and ncRNAs directly targeted by HBx and led to the identification of new mechanisms by which HBx positively regulates cccDNA transcription and HBV replication.

SUBMITTER: Guerrieri F

PROVIDER: S-EPMC5316204 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Publications

Genome-wide identification of direct HBx genomic targets.

Guerrieri Francesca F Belloni Laura L D'Andrea Daniel D Pediconi Natalia N Le Pera Loredana L Testoni Barbara B Scisciani Cecilia C Floriot Oceane O Zoulim Fabien F Tramontano Anna A Levrero Massimo M

BMC genomics 20170217 1

<h4>Background</h4>The Hepatitis B Virus (HBV) HBx regulatory protein is required for HBV replication and involved in HBV-related carcinogenesis. HBx interacts with chromatin modifying enzymes and transcription factors to modulate histone post-translational modifications and to regulate viral cccDNA transcription and cellular gene expression. Aiming to identify genes and non-coding RNAs (ncRNAs) directly targeted by HBx, we performed a chromatin immunoprecipitation sequencing (ChIP-Seq) to analy ...[more]

PMID: 28212627

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Dataset Information

Genome-wide identification of direct HBx genomic targets.

Background

Results

Conclusions

Publications

Genome-wide identification of direct HBx genomic targets.

Similar Datasets

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