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Reliable microfluidic on-chip incubation of droplets in delay-lines.

ABSTRACT: Together with droplet creation, fusion and sorting, the incubation of droplets is one of the most important and essential operations for droplet-based microfluidic assays. This manuscript concerns the development of delay-lines, which are necessary to allow incubation of reactions for precise time periods. We analyze the problems associated with creating delay-lines for incubation in the minute to hour time range, which arise from back-pressure and from the dispersion in the incubation time due to the unequal speeds with which droplets pass through the delay-line. We describe delay-line systems which resolve these problems and demonstrate their use to measure reaction kinetics over several minutes in droplets.

SUBMITTER: Frenz L

PROVIDER: S-EPMC5317046 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Reliable microfluidic on-chip incubation of droplets in delay-lines.

Frenz Lucas L Blank Kerstin K Brouzes Eric E Griffiths Andrew D AD

Lab on a chip 20081219 10

Together with droplet creation, fusion and sorting, the incubation of droplets is one of the most important and essential operations for droplet-based microfluidic assays. This manuscript concerns the development of delay-lines, which are necessary to allow incubation of reactions for precise time periods. We analyze the problems associated with creating delay-lines for incubation in the minute to hour time range, which arise from back-pressure and from the dispersion in the incubation time due ...[more]

PMID: 19417899

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