3D Time-lapse Imaging and Quantification of Mitochondrial Dynamics.

ABSTRACT: We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopolymer coating contains multiple functional groups which impart better cellular uptake and mitochondria targeting efficiency. The high stability of the gold nanoparticles allows continuous imaging over an extended time up to 3000 seconds without significant cell damage. By combining temporal autocorrelation analysis with a classical diffusion model, we quantify mitochondrial dynamics and cast these results into 3D maps showing the heterogeneity of diffusion parameters across the whole cell volume.

SUBMITTER: Sison M 

PROVIDER: S-EPMC5322395 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature