Rapidly evolving homing CRISPR barcodes.
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ABSTRACT:
SUBMITTER: Kalhor R
PROVIDER: S-EPMC5322472 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Publications
Rapidly evolving homing CRISPR barcodes.
Kalhor Reza R Mali Prashant P Church George M GM
Nature methods 20161205 2
We present an approach for engineering evolving DNA barcodes in living cells. A homing guide RNA (hgRNA) scaffold directs the Cas9-hgRNA complex to the DNA locus of the hgRNA itself. We show that this homing CRISPR-Cas9 system acts as an expressed genetic barcode that diversifies its sequence and that the rate of diversification can be controlled in cultured cells. We further evaluate these barcodes in cell populations and show that they can be used to record lineage history and that the barcode ...[more]
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