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Mitochondrial Complex V ? Subunit Is Critical for Candida albicans Pathogenicity through Modulating Multiple Virulence Properties.

ABSTRACT: The ? subunit (ATP1) is a vital component of mitochondrial complex V which counts for the majority of cellular ATP production in a living organism. Nevertheless, how the ? subunit influences other cellular processes such as pathogenicity in Candida albicans remains poorly understood. To address this question, ATP1 mutant (atp1?/?) and the gene-reconstituted strain (atp1?/ATP1) have been constructed in this study and their pathogenicity-related traits are compared to those of wild type (WT). In a murine model of disseminated candidiasis, atp1?/? infected mice have a significantly higher survival rate and experience a lower fungal burden in tissues. In in vitro studies atp1?/? lose a capability to damage or destroy macrophages and endothelial cells. Furthermore, atp1?/? is not able to grow under either glucose-denial conditions or high H₂O₂ conditions, both of which are associated with the potency of the macrophages to kill C. albicans. Defects in filamentation and biofilm formation may impair the ability of atp1?/? to penetrate host cells and establish robust colonies in the host tissues. In concert with these pathogenic features, intracellular ATP levels of atp1?/? can drop to 1/3 of WT level. These results indicate that the ? subunit of Complex V play important roles in C. albicans pathogenicity.

SUBMITTER: Li SX

PROVIDER: S-EPMC5322696 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Mitochondrial Complex V α Subunit Is Critical for Candida albicans Pathogenicity through Modulating Multiple Virulence Properties.

Li Shui-Xiu SX Song Yan-Jun YJ Zhang Yi-Shan YS Wu Hao-Tian HT Guo Hui H Zhu Kun-Ju KJ Li Dong-Mei DM Zhang Hong H

Frontiers in microbiology 20170223

The α subunit (ATP1) is a vital component of mitochondrial complex V which counts for the majority of cellular ATP production in a living organism. Nevertheless, how the α subunit influences other cellular processes such as pathogenicity in Candida albicans remains poorly understood. To address this question, ATP1 mutant (atp1Δ/Δ) and the gene-reconstituted strain (atp1Δ/ATP1) have been constructed in this study and their pathogenicity-related traits are ...[more]

PMID: 28280492

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Dataset Information

Mitochondrial Complex V ? Subunit Is Critical for Candida albicans Pathogenicity through Modulating Multiple Virulence Properties.

Publications

Mitochondrial Complex V α Subunit Is Critical for <i>Candida albicans</i> Pathogenicity through Modulating Multiple Virulence Properties.

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