Ontology highlight
ABSTRACT:
SUBMITTER: Ho IY
PROVIDER: S-EPMC5322767 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Journal of immunological methods 20160904
Generating monoclonal antibodies from single B cells is a valuable tool for characterizing the specificity and functional properties of humoral responses. We and others developed protocols that have facilitated major advances in our understanding of B cell development, tolerance, and effector responses to HIV and influenza. Here, we demonstrate various refinements and dramatically reduce the time required to produce recombinant antibodies. Further, we present new methods for cloning and isolatin ...[more]