Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica.
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ABSTRACT:
SUBMITTER: Celinska E
PROVIDER: S-EPMC5328822 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
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Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica.
Celińska Ewelina E Ledesma-Amaro Rodrigo R Larroude Macarena M Rossignol Tristan T Pauthenier Cyrille C Nicaud Jean-Marc JM
Microbial biotechnology 20170219 2
In this study, we have adopted Golden Gate modular cloning strategy to develop a robust and versatile DNA assembly platform for the nonconventional yeast Yarrowia lipolytica. To this end, a broad set of destination vectors and interchangeable building blocks have been constructed. The DNA modules were assembled on a scaffold of predesigned 4 nt overhangs covering three transcription units (each bearing promoter, gene and terminator), selection marker gene and genomic integration targeting sequen ...[more]
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