Procedures for cryogenic X-ray ptychographic imaging of biological samples.

ABSTRACT: Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.

SUBMITTER: Yusuf M 

PROVIDER: S-EPMC5330525 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature