Ontology highlight
ABSTRACT:
SUBMITTER: Jones CP
PROVIDER: S-EPMC5330840 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Jones Cheryl P CP Khan Kirin K Ingram-Smith Cheryl C
FEBS letters 20170209 4
ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of enzyme variants of Entamoeba ACD in which the two proposed phosphorylated His residues were individually altered revealed that only His252 is essential for enzymatic activity. Anal ...[more]