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Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation.

ABSTRACT: High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data descriptor contains a confocal fluorescence, microscopic image dataset. We also describe an open source, automated image analysis workflow that can be reused to perform high-content analysis of other organelles. This dataset is suitable for analysis of morphological parameters that are linked to human umbilical vein endothelial cell (HUVEC) biology.

SUBMITTER: Ketteler R

PROVIDER: S-EPMC5332014 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation.

Ketteler Robin R Freeman Jamie J Ferraro Francesco F Bata Nicole N Cutler Dan F DF Kriston-Vizi Janos J Stevenson Nicola N

Scientific data 20170301

High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data descriptor contains a confocal fluorescence, microscopic image dataset. We also describe an open source, automated image analysis workflow that can be reused to perform high-content analysis of other ...[more]

PMID: 28248923

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