Dataset Information

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO₂ nanocomposites to suppress reactive oxygen species.

ABSTRACT: Reducing oxidative stress (ROS) have been demonstrated effective for steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH). Selenium (Se) plays an important role in suppressing oxidative stress and has huge potential in ONFH treatments. However the Se has a narrow margin between beneficial and toxic effects which make it hard for therapy use in vivo. In order to make the deficiency up, a control release of Se (Se@SiO₂) were realized by nanotechnology modification. Porous Se@SiO₂ nanocomposites have favorable biocompatibility and can reduced the ROS damage effectively. In vitro, the cck-8 analysis, terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) stain and flow cytometry analysis showed rare negative influence by porous Se@SiO₂ nanocomposites but significantly protective effect against H₂O₂ by reducing ROS level (detected by DCFH-DA). In vivo, the biosafety of porous Se@SiO₂ nanocomposites were confirmed by the serum biochemistry, the ROS level in serum were significantly reduced and the curative effect were confirmed by Micro CT scan, serum Elisa assay (inflammatory factors), Western blotting (quantitative measurement of ONFH) and HE staining. It is expected that the porous Se@SiO₂ nanocomposites may prevent steroid-induced ONFH by reducing oxidative stress.

SUBMITTER: Deng G

PROVIDER: S-EPMC5335566 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO<sub>2</sub> nanocomposites to suppress reactive oxygen species.

Deng Guoying G Niu Kerun K Zhou Feng F Li Buxiao B Kang Yingjie Y Liu Xijian X Hu Junqing J Li Bo B Wang Qiugen Q Yi Chengqing C Wang Qian Q

Scientific reports 20170303

Reducing oxidative stress (ROS) have been demonstrated effective for steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH). Selenium (Se) plays an important role in suppressing oxidative stress and has huge potential in ONFH treatments. However the Se has a narrow margin between beneficial and toxic effects which make it hard for therapy use in vivo. In order to make the deficiency up, a control release of Se (Se@SiO<sub>2</sub>) were realized by nanotechnology modification. P ...[more]

PMID: 28256626

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Project description:PurposeSteroid-induced necrosis of the femoral head (SONFH) is a refractory orthopedic hip disease occurring in young and middle-aged people, with glucocorticoids being the most common cause. Previous experimental studies have shown that cell pyroptosis may be involved in the pathological process of SONFH, but its pathogenesis in SONFH is still unclear. This study aims to screen and validate potential pyroptosis-related genes in SONFH diagnosis by bioinformatics analysis to further elucidate the mechanism of pyroptosis in SONFH.MethodsThere were 33 pyroptosis-related genes obtained from the prior reviews. The mRNA expression was downloaded from GSE123568 dataset in the Gene Expression Omnibus (GEO) database, including 10 non-SONFH (following steroid administration) samples and 30 SONFH samples. The pyroptosis-related differentially expressed genes involved in SONFH were identified with "affy" and "limma" R package by intersecting the GSE123568 dataset with pyroptosis genes. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the pyroptosis-related differentially expressed genes involved in SONFH were conducted by "clusterProfiler" R package and visualized by "GOplot" R package. Then, the correlations between the expression levels of the pyroptosis-related differentially expressed genes involved in SONFH were confirmed with "corrplot" R package. Moreover, the protein-protein interaction (PPI) network was analysed by using GeneMANIA database. Next, The ROC curve of pyroptosis-related differentially expressed genes were analyzed by "pROC" R package.ResultsA total of 10 pyroptosis-related differentially expressed genes were identified between the peripheral blood samples of SONFH patients and non-SONFH patients based on the defined criteria, including 20 upregulated genes and 10 downregulated genes. The GO and KEGG pathway enrichment analyses revealed that these 10 pyroptosis-related differentially expressed genes involved in SONFH were particularly enriched in cysteine-type endopeptidase activity involved in apoptotic process, positive regulation of interleukin-1 beta secretion and NOD-like receptor signaling pathway. Correlation analysis revealed significant correlations among the 10 differentially expressed pyroptosis-related genes involved in SONFH. The PPI results demonstrated that the 10 pyroptosis-related differentially expressed genes interacted with each other. Compared to non-SONFH samples, these pyroptosis-related differentially expressed genes had good predictive diagnostic efficacy (AUC = 1.000, CI = 1.000-1.000) in the SONFH samples, and NLRP1 had the highest diagnostic value (AUC: 0.953) in the SONFH samples.ConclusionsThere were 10 potential pyroptosis-related differentially expressed genes involved in SONFH were identified via bioinformatics analysis, which might serve as potential diagnostic biomarkers because they regulated pyroptosis. These results expand the understanding of SONFH associated with pyroptosis and provide new insights to further explore the mechanism of action and diagnosis of pyroptosis associated in SONFH.

Dataset Information

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO₂ nanocomposites to suppress reactive oxygen species.

Publications

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO<sub>2</sub> nanocomposites to suppress reactive oxygen species.

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Dataset Information

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species.

Publications

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO<sub>2</sub> nanocomposites to suppress reactive oxygen species.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO₂ nanocomposites to suppress reactive oxygen species.