Protein-ligand binding affinity determination by the waterLOGSY method: An optimised approach considering ligand rebinding.
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ABSTRACT:
SUBMITTER: Huang R
PROVIDER: S-EPMC5335602 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
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Protein-ligand binding affinity determination by the waterLOGSY method: An optimised approach considering ligand rebinding.
Huang Renjie R Bonnichon Arnaud A Claridge Timothy D W TD Leung Ivanhoe K H IK
Scientific reports 20170303
WaterLOGSY is a popular ligand-observed NMR technique to screen for protein-ligand interactions, yet when applied to measure dissociation constants (K<sub>D</sub>) through ligand titration, the results were found to be strongly dependent on sample conditions. Herein, we show that accurate K<sub>D</sub>s can be obtained by waterLOGSY with optimised experimental setup. ...[more]
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