Unknown

Dataset Information

0

MiR-125b-5p enhances chemotherapy sensitivity to cisplatin by down-regulating Bcl2 in gallbladder cancer.


ABSTRACT: Gallbladder cancer represents the most common malignancy of the biliary tract and is highly lethal with less than 5% overall 5-year survival rate. Chemotherapy remains the major treatment for late-stage patients. However, insensitivity to these chemotherapeutic agents including cisplatin is common. MicroRNAs (miRNAs) have been shown as modulators of drug resistance in many cancer types. We used genome-wide gene expression analysis in clinical samples to identify miR-125b-5p down-regulated in gallbladder cancer. miR-125b-5p up-regulation promoted cell death in gallbladder cancer cells in the presence of cisplatin. In contrast, knockdown of miR-125b-5p reduced cell death in gallbladder cancer cells treated with cisplatin. Up-regulation of miR-125b-5p significantly decreased tumor growth in combination with cisplatin in a mouse model. We identified Bcl2 as a direct target of miR-125b-5p which mediates the function of miR-125b-5p in gallbladder cancer. In clinical samples, miR-125b-5p was down-regulated in gallbladder cancer whereas Bcl2 was up-regulated and their expression was inversely correlated. Moreover, low miR-125b-5p expression or high expression of Bcl2 is correlated with poor prognosis in gallbladder cancer. Taken together, our findings indicate that miR-125b-5p is a potent chemotherapy sensitizer and may function as a new biomarker for the prognosis of gallbladder cancer patients.

SUBMITTER: Yang D 

PROVIDER: S-EPMC5335654 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

miR-125b-5p enhances chemotherapy sensitivity to cisplatin by down-regulating Bcl2 in gallbladder cancer.

Yang Dong D   Zhan Ming M   Chen Tao T   Chen Wei W   Zhang Yunhe Y   Xu Sunwang S   Yan Jinchun J   Huang Qihong Q   Wang Jian J  

Scientific reports 20170303


Gallbladder cancer represents the most common malignancy of the biliary tract and is highly lethal with less than 5% overall 5-year survival rate. Chemotherapy remains the major treatment for late-stage patients. However, insensitivity to these chemotherapeutic agents including cisplatin is common. MicroRNAs (miRNAs) have been shown as modulators of drug resistance in many cancer types. We used genome-wide gene expression analysis in clinical samples to identify miR-125b-5p down-regulated in gal  ...[more]

Similar Datasets

| S-EPMC5999287 | biostudies-literature
| S-EPMC8748520 | biostudies-literature
| S-EPMC5739794 | biostudies-literature
| S-EPMC8025462 | biostudies-literature
| S-EPMC4886683 | biostudies-other
2021-01-21 | GSE165183 | GEO
| S-EPMC6606789 | biostudies-literature
| S-EPMC7533283 | biostudies-literature
| S-EPMC9281112 | biostudies-literature
| S-EPMC8082674 | biostudies-literature