The vaccinia virus K7 protein promotes histone methylation associated with heterochromatin formation.

ABSTRACT: It has been well established that many vaccinia virus proteins suppress host antiviral pathways by targeting the transcription of antiviral proteins, thus evading the host innate immune system. However, whether viral proteins have an effect on the host's overall cellular transcription is less understood. In this study we investigated the regulation of heterochromatin during vaccinia virus infection. Heterochromatin is a highly condensed form of chromatin that is less transcriptionally active and characterized by methylation of histone proteins. We examined the change in methylation of two histone proteins, H3 and H4, which are major markers of heterochromatin, during the course of viral infection. Using immunofluorescence microscopy and flow cytometry we were able to track the overall chan

SUBMITTER: Teferi WM 

PROVIDER: S-EPMC5336242 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature