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A new malaria vector mosquito in South Africa.

ABSTRACT: South Africa aims to eliminate malaria within its borders by 2018. Despite well-coordinated provincial vector control programmes that are based on indoor residual insecticide spraying, low-level residual malaria transmission continues in the low-altitude border regions of the north-eastern sector of the country. In order to identify the underlying causes of residual transmission, an enhanced vector surveillance system has been implemented at selected sites in the Mpumalanga and KwaZulu-Natal (KZN) provinces. The collection periods for the data presented are March 2015 to April 2016 for Mpumalanga and January 2014 to December 2015 for KZN. The mosquito collection methods used included indoor and outdoor traps based on the use of traditional ceramic pots, modified plastic buckets and exit window traps (KZN only). All Anopheles funestus species group mosquitoes collected were identified to species and all females were screened for the presence of Plasmodium falciparum sporozoites. Two An. vaneedeni females, one from each surveillance site, tested positive for P. falciparum sporozoites. These are the first records of natural populations of An. vaneedeni being infective with P. falciparum. As both specimens were collected from outdoor-placed ceramic pots, these data show that An. vaneedeni likely contributes to residual malaria transmission in South Africa.

SUBMITTER: Burke A

PROVIDER: S-EPMC5338325 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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A new malaria vector mosquito in South Africa.

Burke Ashley A Dandalo Leonard L Munhenga Givemore G Dahan-Moss Yael Y Mbokazi Frans F Ngxongo Sifiso S Coetzee Maureen M Koekemoer Lizette L Brooke Basil B

Scientific reports 20170306

South Africa aims to eliminate malaria within its borders by 2018. Despite well-coordinated provincial vector control programmes that are based on indoor residual insecticide spraying, low-level residual malaria transmission continues in the low-altitude border regions of the north-eastern sector of the country. In order to identify the underlying causes of residual transmission, an enhanced vector surveillance system has been implemented at selected sites in the Mpumalanga and KwaZulu-Natal (KZ ...[more]

PMID: 28262811

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Project description:Human Malaria is transmitted by mosquitoes of the genus Anopheles. Transmission is a complex phenomenon involving biological and environmental factors of humans, parasites and mosquitoes. Among more than 500 anopheline species, only a few species from different branches of the mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. Anopheles albimanus (subgenus Nyssorhynchus) is an important malaria vector in the Americas. The divergence time between Anopheles gambiae, the main malaria vector in Africa, and the Neotropical vectors has been estimated to be 100 My. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to explore the mosquito biology beyond the An. gambiae complex.We sequenced the transcriptome of the An. albimanus adult female. By combining Sanger, 454 and Illumina sequences from cDNA libraries derived from the midgut, cuticular fat body, dorsal vessel, salivary gland and whole body, we generated a single, high-quality assembly containing 16,669 transcripts, 92% of which mapped to the An. darlingi genome and covered 90% of the core eukaryotic genome. Bidirectional comparisons between the An. gambiae, An. darlingi and An. albimanus predicted proteomes allowed the identification of 3,772 putative orthologs. More than half of the transcripts had a match to proteins in other insect vectors and had an InterPro annotation. We identified several protein families that may be relevant to the study of Plasmodium-mosquito interaction. An open source transcript annotation browser called GDAV (Genome-Delinked Annotation Viewer) was developed to facilitate public access to the data generated by this and future transcriptome projects.We have explored the adult female transcriptome of one important New World malaria vector, An. albimanus. We identified protein-coding transcripts involved in biological processes that may be relevant to the Plasmodium lifecycle and can serve as the starting point for searching targets for novel control strategies. Our data increase the available genomic information regarding An. albimanus several hundred-fold, and will facilitate molecular research in medical entomology, evolutionary biology, genomics and proteomics of anopheline mosquito vectors. The data reported in this manuscript is accessible to the community via the VectorBase website (http://www.vectorbase.org/Other/AdditionalOrganisms/).

Project description:Small laboratory cage trials of non-drive and gene-drive strains of the Asian malaria vector mosquito, Anopheles stephensi, were used to investigate release ratios and other strain properties for their impact on transgene spread during simulated population modification. We evaluated the effects of transgenes on survival, male contributions to next-generation populations, female reproductive success and the impact of accumulation of gene drive-resistant genomic target sites resulting from nonhomologous end-joining (NHEJ) mutagenesis during Cas9, guide RNA-mediated cleavage. Experiments with a non-drive, autosomally-linked malaria-resistance gene cassette showed 'full introduction' (100% of the insects have at least one copy of the transgene) within 8 weeks (? 3 generations) following weekly releases of 10:1 transgenic:wild-type males in an overlapping generation trial design. Male release ratios of 1:1 resulted in cages where mosquitoes with at least one copy of the transgene fluctuated around 50%. In comparison, two of three cages in which the malaria-resistance genes were linked to a gene-drive system in an overlapping generation, single 1:1 release reached full introduction in 6-8 generations with a third cage at ~80% within the same time. Release ratios of 0.1:1 failed to establish the transgenes. A non-overlapping generation, single-release trial of the same gene-drive strain resulted in two of three cages reaching 100% introduction within 6-12 generations following a 1:1 transgenic:wild-type male release. Two of three cages with 0.33:1 transgenic:wild-type male single releases achieved full introduction in 13-16 generations. All populations exhibiting full introduction went extinct within three generations due to a significant load on females having disruptions of both copies of the target gene, kynurenine hydroxylase. While repeated releases of high-ratio (10:1) non-drive constructs could achieve full introduction, results from the 1:1 release ratios across all experimental designs favor the use of gene drive, both for efficiency and anticipated cost of the control programs.

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A new malaria vector mosquito in South Africa.

Publications

A new malaria vector mosquito in South Africa.

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