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The Eph-related tyrosine kinase ligand Ephrin-B1 marks germinal center and memory precursor B cells.


ABSTRACT: Identification of germinal center (GC) B cells is typically reliant on the use of surface activation markers that exhibit a wide range of expression. Here, we identify Ephrin-B1, a ligand for Eph-related receptor tyrosine kinases, as a specific marker of mature GC B cells. The number of Ephrin-B1+ GC B cells increases during the course of an immune response with Ephrin-B1+ GC B cells displaying elevated levels of Bcl6, S1pr2, and Aicda relative to their Ephrin-B1- counterparts. We further identified a small proportion of recently dividing, somatically mutated Ephrin-B1+ GC B cells that have begun to down-regulate Bcl6 and S1pr2 and express markers associated with memory B cells, such as CD38 and EBI2. Transcriptional analysis indicates that these cells are developmentally related to memory B cells, and likely represent a population of GC memory precursor (PreMem) B cells. GC PreMem cells display enhanced survival relative to bulk GC B cells, localize near the edge of the GC, and are predominantly found within the light zone. These findings offer insight into the significant heterogeneity that exists within the GC B cell population and provide tools to further dissect signals regulating the differentiation of GC B cells.

SUBMITTER: Laidlaw BJ 

PROVIDER: S-EPMC5339677 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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The Eph-related tyrosine kinase ligand Ephrin-B1 marks germinal center and memory precursor B cells.

Laidlaw Brian J BJ   Schmidt Timothy H TH   Green Jesse A JA   Allen Christopher D C CD   Okada Takaharu T   Cyster Jason G JG  

The Journal of experimental medicine 20170131 3


Identification of germinal center (GC) B cells is typically reliant on the use of surface activation markers that exhibit a wide range of expression. Here, we identify Ephrin-B1, a ligand for Eph-related receptor tyrosine kinases, as a specific marker of mature GC B cells. The number of Ephrin-B1<sup>+</sup> GC B cells increases during the course of an immune response with Ephrin-B1<sup>+</sup> GC B cells displaying elevated levels of Bcl6, <i>S1pr2</i>, and <i>Aicda</i> relative to their Ephrin  ...[more]

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