Ontology highlight
ABSTRACT:
SUBMITTER: de Jong NW
PROVIDER: S-EPMC5339689 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
de Jong Nienke W M NW van der Horst Thijs T van Strijp Jos A G JA Nijland Reindert R
Scientific reports 20170307
We present integration vectors for Staphylococcus aureus encoding the fluorescent reporters mAmetrine, CFP, sGFP, YFP, mCherry and mKate. The expression is driven either from the sarA-P1 promoter or from any other promoter of choice. The reporter can be inserted markerless in the chromosome of a wide range of S. aureus strains. The integration site chosen does not disrupt any open reading frame, provides good expression, and has no detectable effect on the strains physiology. As an intermediate ...[more]