Dataset Information

Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials.

ABSTRACT: Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals. Expression of the cloned H5 gene was achieved using the nisin-controlled gene expression system. Detection of the intracellular H5 antigen produced in L. lactis was performed by Western blot analysis and confirmed using mass spectrometry. The potential of L. lactis recombinant cells to induce an immune response was examined by setting up preliminary immunization trials on chickens and mice. Obtained sera were tested for specific antibodies by ELISA assays. The results of these studies are a promising step toward developing a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for efficient antigen production and delivery to the mucosal surface.

SUBMITTER: Szczepankowska AK

PROVIDER: S-EPMC5340954 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials.

Szczepankowska Agnieszka K AK Szatraj Katarzyna K Sałański Przemysław P Rózga Agnieszka A Górecki Roman K RK Bardowski Jacek K JK

BioMed research international 20170222

Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expr ...[more]

PMID: 28321412

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Project description:Avian pathogenic Escherichia coli (APEC) infections result in significant economic losses and reduced animal welfare. Historically, antibiotics and vaccinations currently control APEC infections in poultry, however, antibiotic-resistant strains and heterologous serotypes limit their effectiveness. Meanwhile, antibiotic-resistant strains can be transmitted to humans via contact with animals, food or their environment. Probiotics and antimicrobial peptides (AMPs) are potential alternatives to antibiotics and represent promising strategies to combat APEC. Bovine lactoferricin and lactoferrampin possess anti-bacterial, anti-inflammatory, and anti-oxidant properties. Lactococcus lactis (L. lactis) is an excellent vector for delivering recombinant proteins. In this research, we generated a recombinant L. lactis strain MG1363 expressing lactoferrin peptides, which was labeled with a fluorescent marker mCherry and lacked an antibiotic resistance gene (LL-EFLmC). Our investigation focused on the impact of LL-EFLmC strain on the gut microbiota composition and avian pathogenic E. coli O78 challenge. Our findings indicate that LL-EFLmC exhibits inhibitory effects against APEC-O78 and Staphylococcus aureus CVCC26003 (S. aureus CVCC26003) in vitro. Furthermore, the inclusion of LL-EFLmC in chicken feed significantly improved the average daily intake and gain to feed ratio. Additionally, LL-EFLmC treatment resulted in a significant increase in serum IgG and intestinal mucus SIgA levels. Administration of LL-EFLmC was found to effectively suppress APEC-O78 infection and mitigate the expression of pro-inflammatory cytokines, including IL-1β, IL-12, IFN-γ, and TNF-α. Additionally, 16S rDNA sequencing data revealed that LL-EFLmC was able to restore the intestinal flora that had been disrupted by APEC-O78. These findings suggest that LL-EFLmC may serve as a promising feed additive and antibiotic alternative in chicken production, due to its potential to enhance immune regulation, promote growth, and confer resistance against APEC-O78 infection.

Dataset Information

Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials.

Publications

Recombinant <i>Lactococcus lactis</i> Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials.

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