Ontology highlight
ABSTRACT:
SUBMITTER: Omi N
PROVIDER: S-EPMC5341036 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Omi Natsue N Tokuda Yuichi Y Ikeda Yoko Y Ueno Morio M Mori Kazuhiko K Sotozono Chie C Kinoshita Shigeru S Nakano Masakazu M Tashiro Kei K
Scientific reports 20170308
Lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus (EBV) serve as an unlimited resource of human genomic DNA. The protocol that is widely used to establish LCLs involves peripheral blood mononuclear cell isolation by density gradient centrifugation, however, that method requires as much as 5 ml of peripheral blood. In this study, in order to provide a more simple and efficient method for the generation of LCLs, we developed a new protocol using hemolytic reaction to enrich white ...[more]