Ontology highlight
ABSTRACT:
SUBMITTER: Popovic A
PROVIDER: S-EPMC5341072 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Popovic Ana A Hai Tran T Tchigvintsev Anatoly A Hajighasemi Mahbod M Nocek Boguslaw B Khusnutdinova Anna N AN Brown Greg G Glinos Julia J Flick Robert R Skarina Tatiana T Chernikova Tatyana N TN Yim Veronica V Brüls Thomas T Paslier Denis Le DL Yakimov Michail M MM Joachimiak Andrzej A Ferrer Manuel M Golyshina Olga V OV Savchenko Alexei A Golyshin Peter N PN Yakunin Alexander F AF
Scientific reports 20170308
Metagenomics has made accessible an enormous reserve of global biochemical diversity. To tap into this vast resource of novel enzymes, we have screened over one million clones from metagenome DNA libraries derived from sixteen different environments for carboxylesterase activity and identified 714 positive hits. We have validated the esterase activity of 80 selected genes, which belong to 17 different protein families including unknown and cyclase-like proteins. Three metagenomic enzymes exhibit ...[more]