High-efficient production and biophysical characterisation of nicastrin and its interaction with APPC100.
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ABSTRACT:
SUBMITTER: Yu K
PROVIDER: S-EPMC5343570 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
High-efficient production and biophysical characterisation of nicastrin and its interaction with APPC100.
Yu Kun K Yang Ge G Labahn Jörg J
Scientific reports 20170309
Nicastrin, the largest member among the four components of the γ-secretase complex, has been identified to be the substrate recognizer for the proteolytic activity of the complex. Here we report that full-length human nicastrin (hNCT) can be obtained by heterologous expression in E. coli. Milligram quantities of the target protein are purified in a two-step purification protocol using affinity chromatography followed by SEC. The FOS-choline 14 purified tetrameric hNCT exhibits a proper folding w ...[more]
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