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Printed covalent glycan array for ligand profiling of diverse glycan binding proteins.

ABSTRACT: Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.

SUBMITTER: Blixt O

PROVIDER: S-EPMC534418 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature

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Printed covalent glycan array for ligand profiling of diverse glycan binding proteins.

Blixt Ola O Head Steve S Mondala Tony T Scanlan Christopher C Huflejt Margaret E ME Alvarez Richard R Bryan Marian C MC Fazio Fabio F Calarese Daniel D Stevens James J Razi Nahid N Stevens David J DJ Skehel John J JJ van Die Irma I Burton Dennis R DR Wilson Ian A IA Cummings Richard R Bovin Nicolai N Wong Chi-Huey CH Paulson James C JC

Proceedings of the National Academy of Sciences of the United States of America 20041124 49

Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine functionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galectins, anticarbohydrate antibodies, le ...[more]

PMID: 15563589

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