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Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.

ABSTRACT: OBJECTIVE:To assess if injection of allogeneic dental pulp tissue particles would improve lameness in horses with naturally occurring osteoarthritis (OA) or soft tissue (ST) injury. DESIGN:Prospective, randomized, blinded, and controlled clinical trial and client survey assessment. ANIMALS:Forty lame client-owned horses. PROCEDURES:Sterile dental pulp, recovered from otherwise healthy foals that perish during dystocia, was processed under good manufacturing processing to produce mechanically manipulated, unexpanded pulp tissue particles containing viable cells surrounded in extracellular matrix. Forty lame client-owned horses with confirmed OA (n?=?20), or ST injury (desmitis or tendonitis) received a 2?mL intra-articular (n?=?20 OA) or intra-lesional (n?=?20) injection of control transport vehicle (n?=?20) or 10?×?106 dental pulp tissue particles (n?=?20). Acclimatized horses had baseline measurements performed and were then injected on day 0. Horses were treadmill exercised for 2?weeks, evaluated by clinical parameters, lameness score, edema (score and circumference), pain on flexion (OA) or pressure (ST), and clients' scores for pain and discomfort before and through 45?days after pulp injection. Twenty horses were available for >2.5-year follow-up. RESULTS:Pulp-treated horses showed decrease in lameness compared to baseline (P?2.5-year follow-up, at least 10 horses were in work. CONCLUSION AND CLINICAL RELEVANCE:Dental pulp tissue particles can be considered as a treatment option for equine lameness due to OA, desmitis, or tendonitis.

SUBMITTER: Bertone AL

PROVIDER: S-EPMC5344919 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.

Bertone Alicia L AL Reisbig Nathalie A NA Kilborne Allison H AH Kaido Mari M Salmanzadeh Navid N Lovasz Rebecca R Sizemore Joy L JL Scheuermann Logan L Kopp Rosalind J RJ Zekas Lisa J LJ Brokken Matthew T MT

Frontiers in veterinary science 20170310

<h4>Objective</h4>To assess if injection of allogeneic dental pulp tissue particles would improve lameness in horses with naturally occurring osteoarthritis (OA) or soft tissue (ST) injury.<h4>Design</h4>Prospective, randomized, blinded, and controlled clinical trial and client survey assessment.<h4>Animals</h4>Forty lame client-owned horses.<h4>Procedures</h4>Sterile dental pulp, recovered from otherwise healthy foals that perish during dystocia, was processed under good manufacturing processin ...[more]

PMID: 28344975

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Project description:Pulp engineering with dental mesenchymal stem cells is a promising therapy for injured teeth. An important point is to determine the fate of implanted cells in the pulp over time and particularly during the early phase following implantation. Indeed, the potential engraftment of the implanted cells in other organs has to be assessed, in particular, to evaluate the risk of inducing ectopic mineralization. In this study, our aim was to follow by nuclear imaging the radiolabeled pulp cells after implantation in the rat emptied pulp chamber. For that purpose, indium-111-oxine (¹¹¹In-oxine)-labeled rat pulp cells were added to polymerizing type I collagen hydrogel to obtain a pulp equivalent. This scaffold was implanted in the emptied pulp chamber space in the upper first rat molar. Labeled cells were then tracked during 3 weeks by helical single-photon emission computed tomography (SPECT)/computed tomography performed on a dual modality dedicated small animal camera. Negative controls were performed using lysed radiolabeled cells obtained in a hypotonic solution. In vitro data indicated that ¹¹¹In-oxine labeling did not affect cell viability and proliferation. In vivo experiments allowed a noninvasive longitudinal follow-up of implanted living cells for at least 3 weeks and indicated that SPECT signal intensity was related to implanted cell integrity. Notably, there was no detectable systemic release of implanted cells from the tooth. In addition, histological analysis of the samples showed mitotically active fibroblastic cells as well as neoangiogenesis and nervous fibers in pulp equivalents seeded with entire cells, whereas pulp equivalents prepared from lysed cells were devoid of cell colonization. In conclusion, our study demonstrates that efficient labeling of pulp cells can be achieved and, for the first time, that these cells can be followed up after implantation in the tooth by nuclear imaging. Furthermore, it appears that grafted cells retained the label and are viable to follow the repair process. This technique is expected to be of major interest for monitoring implanted cells in innovative therapies for injured teeth.

Dataset Information

Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.

Publications

Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.

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