Ontology highlight
ABSTRACT:
SUBMITTER: Yanez J
PROVIDER: S-EPMC534637 | biostudies-literature | 2004 Nov
REPOSITORIES: biostudies-literature
Yáñez Jorge J Argüello Martha M Osuna Joel J Soberón Xavier X Gaytán Paul P
Nucleic acids research 20041110 20
Twenty Fmoc-protected trinucleotide phosphoramidites representing a complete set of codons for the natural amino acids were chemically synthesized for the first time. A pool of these reagents was incorporated into oligonucleotides at substoichiometric levels to generate two libraries of variants that randomly carry either few or many codon replacements on a region encoding nine amino acids of the bacterial enzyme TEM-1 beta-lactamase. Assembly of the libraries was performed in a completely autom ...[more]