Ontology highlight
ABSTRACT:
SUBMITTER: Valenzuela-Chavira I
PROVIDER: S-EPMC5346462 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Valenzuela-Chavira Ignacio I Contreras-Vergara Carmen A CA Arvizu-Flores Aldo A AA Serrano-Posada Hugo H Lopez-Zavala Alonso A AA García-Orozco Karina D KD Hernandez-Paredes Javier J Rudiño-Piñera Enrique E Stojanoff Vivian V Sotelo-Mundo Rogerio R RR Islas-Osuna Maria A MA
Biochimie 20170117
We studied a mango glutathione S-transferase (GST) (Mangifera indica) bound to glutathione (GSH) and S-hexyl glutathione (GSX). This GST Tau class (MiGSTU) had a molecular mass of 25.5 kDa. MiGSTU Michaelis-Menten kinetic constants were determined for their substrates obtaining a K<sub>m</sub>, V<sub>max</sub> and k<sub>cat</sub> for CDNB of 0.792 mM, 80.58 mM min<sup>-1</sup> and 68.49 s<sup>-1</sup> respectively and 0.693 mM, 105.32 mM min<sup>-1</sup> and 89.57 s<sup>-1</sup>, for reduced GSH ...[more]