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IL-17A-mediated neutrophil recruitment limits expansion of segmented filamentous bacteria.


ABSTRACT: Specific components of the intestinal microbiota are capable of influencing immune responses such that a mutualistic relationship is established. In mice, colonization with segmented filamentous bacteria (SFB) induces T-helper-17 (Th17) cell differentiation in the intestine, yet the effector functions of interleukin (IL)-17A in response to SFB remain incompletely understood. Here we report that colonization of mice with SFB-containing microbiota induced IL-17A- and CXCR2-dependent recruitment of neutrophils to the ileum. This response required adaptive immunity, as Rag-deficient mice colonized with SFB-containing microbiota failed to induce IL-17A, CXCL1 and CXCL2, and displayed defective neutrophil recruitment to the ileum. Interestingly, neutrophil depletion in wild-type mice resulted in significantly augmented Th17 responses and SFB expansion, which correlated with impaired expression of IL-22 and antimicrobial peptides. These data provide novel insight into a dynamic IL-17A-CXCR2-neutrophil axis during acute SFB colonization and demonstrate a central role for neutrophils in limiting SFB expansion.

SUBMITTER: Flannigan KL 

PROVIDER: S-EPMC5350071 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

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IL-17A-mediated neutrophil recruitment limits expansion of segmented filamentous bacteria.

Flannigan K L KL   Ngo V L VL   Geem D D   Harusato A A   Hirota S A SA   Parkos C A CA   Lukacs N W NW   Nusrat A A   Gaboriau-Routhiau V V   Cerf-Bensussan N N   Gewirtz A T AT   Denning T L TL  

Mucosal immunology 20160914 3


Specific components of the intestinal microbiota are capable of influencing immune responses such that a mutualistic relationship is established. In mice, colonization with segmented filamentous bacteria (SFB) induces T-helper-17 (Th17) cell differentiation in the intestine, yet the effector functions of interleukin (IL)-17A in response to SFB remain incompletely understood. Here we report that colonization of mice with SFB-containing microbiota induced IL-17A- and CXCR2-dependent recruitment of  ...[more]

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