Ontology highlight
ABSTRACT:
SUBMITTER: Jung CJ
PROVIDER: S-EPMC5350237 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Transgenic research 20161130 2
The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HDR using mouse embryonic stem cells as a model system. We characterized several parameters, including single guide RNA cleavage activity and the length and symmetry of homology arms in the construct, a ...[more]